Direct regenerationin vitro and transient GUS expression inMentha xpiperita.
Identifieur interne : 000923 ( Main/Exploration ); précédent : 000922; suivant : 000924Direct regenerationin vitro and transient GUS expression inMentha xpiperita.
Auteurs : J C Caissard [France] ; O. Faure ; F. Jullien ; M. Colson ; A. PerrinSource :
- Plant cell reports [ 0721-7714 ] ; 1996.
Abstract
Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is:Agrobacterium inoculation or biolistic treatment of the first apical leaves ofin vitro clones, regeneration in the dark with kanamycin (1 mg l(-1)) and 6-benzylaminopurine (2 mg l(-1)), followed by selection of regenerated shoots with 200 mg 1(-1) kanamycin.
DOI: 10.1007/BF01275452
PubMed: 24178657
Affiliations:
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Perrin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:24178657</idno><idno type="pmid">24178657</idno><idno type="doi">10.1007/BF01275452</idno><idno type="wicri:Area/Main/Corpus">000920</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000920</idno><idno type="wicri:Area/Main/Curation">000920</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000920</idno><idno type="wicri:Area/Main/Exploration">000920</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Direct regenerationin vitro and transient GUS expression inMentha xpiperita.</title><author><name sortKey="Caissard, J C" sort="Caissard, J C" uniqKey="Caissard J" first="J C" last="Caissard">J C Caissard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Biotechnologies Appliquées aux Plantes Aromatiques, Faculté des Sciences et Techniques, Université Jean Monnet, 23 rue du Dr Michelon, 42023, Saint-Etienne Cédex 2, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biotechnologies Appliquées aux Plantes Aromatiques, Faculté des Sciences et Techniques, Université Jean Monnet, 23 rue du Dr Michelon, 42023, Saint-Etienne Cédex 2</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Saint-Etienne Cédex 2</settlement></placeName></affiliation></author><author><name sortKey="Faure, O" sort="Faure, O" uniqKey="Faure O" first="O" last="Faure">O. Faure</name></author><author><name sortKey="Jullien, F" sort="Jullien, F" uniqKey="Jullien F" first="F" last="Jullien">F. Jullien</name></author><author><name sortKey="Colson, M" sort="Colson, M" uniqKey="Colson M" first="M" last="Colson">M. Colson</name></author><author><name sortKey="Perrin, A" sort="Perrin, A" uniqKey="Perrin A" first="A" last="Perrin">A. Perrin</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is:Agrobacterium inoculation or biolistic treatment of the first apical leaves ofin vitro clones, regeneration in the dark with kanamycin (1 mg l(-1)) and 6-benzylaminopurine (2 mg l(-1)), followed by selection of regenerated shoots with 200 mg 1(-1) kanamycin. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24178657</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>16</Volume><Issue>1-2</Issue><PubDate><Year>1996</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>Direct regenerationin vitro and transient GUS expression inMentha xpiperita.</ArticleTitle><Pagination><MedlinePgn>67-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF01275452</ELocationID><Abstract><AbstractText>Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is:Agrobacterium inoculation or biolistic treatment of the first apical leaves ofin vitro clones, regeneration in the dark with kanamycin (1 mg l(-1)) and 6-benzylaminopurine (2 mg l(-1)), followed by selection of regenerated shoots with 200 mg 1(-1) kanamycin. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Caissard</LastName><ForeName>J C</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Laboratoire de Biotechnologies Appliquées aux Plantes Aromatiques, Faculté des Sciences et Techniques, Université Jean Monnet, 23 rue du Dr Michelon, 42023, Saint-Etienne Cédex 2, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Faure</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Jullien</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Colson</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Perrin</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1995</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>1996</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24178657</ArticleId><ArticleId IdType="doi">10.1007/BF01275452</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Saint-Etienne Cédex 2</li></settlement></list><tree><noCountry><name sortKey="Colson, M" sort="Colson, M" uniqKey="Colson M" first="M" last="Colson">M. 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